The DOT Drug and Alcohol Testing Regulations require all CDL drivers (operating a vehicle over 26,000 lbs., carrying 16+ passengers, or hauling hazmat) to be D&A tested under certain circumstances. DOT D&A testing regulations not only affect the drivers, but also a number of responsible parties such as the employer/motor carrier, safety manager or designated employer representative, collectors, substance abuse professionals, the laboratory, the medical review officer, etc.